7OY3
Crystal structure of depupylase Dop in complex with phosphorylated Pup and ADP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-09-10 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.815 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 106.806, 106.806, 108.457 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 46.780 - 1.780 |
R-factor | 0.1711 |
Rwork | 0.170 |
R-free | 0.19360 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5lrt |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.910 | 1.844 |
High resolution limit [Å] | 1.776 | 1.780 |
Number of reflections | 68753 | 6798 |
<I/σ(I)> | 12.56 | |
Completeness [%] | 99.8 | |
Redundancy | 20.6 | |
CC(1/2) | 0.999 | 0.774 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293.15 | 100 mM Tris-acetate pH 7.0-8.5, 10 mM ADP, 40 mM MgCl2 and 0.75-1.0 M KH2PO4 |