7OX1

Fab 7D6: hIL-9 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE MASSIF-3
Synchrotron site	ESRF
Beamline	MASSIF-3
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-10-25
Detector	DECTRIS EIGER X 4M
Wavelength(s)	0.96770
Spacegroup name	P 1 21 1
Unit cell lengths	81.350, 102.750, 164.370
Unit cell angles	90.00, 94.12, 90.00

Refinement procedure

Resolution	42.360 - 2.490
R-factor	0.2118
Rwork	0.209
R-free	0.26200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4hc1 4r90
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.360	2.640
High resolution limit [Å]	2.490	2.490
Number of reflections	92495	14578
<I/σ(I)>	11.99
Completeness [%]	97.7
Redundancy	3.2
CC(1/2)	0.997	0.495

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.25M ammonium nitrate, 18% PEG 3350, 5% ethylene glycol