7OWZ
Heterodimeric murine tRNA-guanine transglycosylase in complex with queuine and in the presence of Anderson-Evans type (TEW) and Strandberg type polyoxometalate (POM)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.1 |
Synchrotron site | BESSY |
Beamline | 14.1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-12-07 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.918400 |
Spacegroup name | P 62 2 2 |
Unit cell lengths | 199.500, 199.500, 90.586 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 48.600 - 2.600 |
R-factor | 0.2151 |
Rwork | 0.214 |
R-free | 0.23940 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6h62 6fv5 |
RMSD bond length | 0.003 |
RMSD bond angle | 1.010 |
Data reduction software | XDS (1.02) |
Data scaling software | XDS (1.02) |
Phasing software | PHASER (7.0.047) |
Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.600 | 2.750 |
High resolution limit [Å] | 2.600 | 2.600 |
Number of reflections | 62234 | 9974 |
<I/σ(I)> | 24.06 | |
Completeness [%] | 99.9 | |
Redundancy | 12.75 | |
CC(1/2) | 1.000 | 0.833 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291.15 | 100 mM Na/K phosphate pH 6.8, 200 mM Sodium chloride, 14 % (w/v) PEG 1000, 1 mM TEW |