7OWY
Crystal structure of human mitochondrial ferritin (hMTF) Fe(II)-loaded for 3 minutes showing a peroxide anion as bridging species of iron ions in the ferroxidase site
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-09-07 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.97950 |
Spacegroup name | F 4 3 2 |
Unit cell lengths | 184.111, 184.111, 184.111 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 55.570 - 1.550 |
R-factor | 0.1536 |
Rwork | 0.153 |
R-free | 0.17220 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1r03 |
RMSD bond length | 0.007 |
RMSD bond angle | 1.377 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 65.090 | 1.630 |
High resolution limit [Å] | 1.550 | 1.550 |
Rmerge | 0.163 | 0.622 |
Rmeas | 0.168 | 0.646 |
Rpim | 0.036 | 0.141 |
Number of reflections | 39259 | 5609 |
<I/σ(I)> | 12 | 4.4 |
Completeness [%] | 100.0 | 100 |
Redundancy | 21.2 | 20.9 |
CC(1/2) | 0.997 | 0.929 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 281.15 | 1.6-2 M MgCl2 6H2O and 0.1 M bicine pH 9.0 |