7OW1

Crystal Structure of TAP01 in complex with amyloid beta peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID30B
Synchrotron site	ESRF
Beamline	ID30B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-04-13
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.968625
Spacegroup name	P 41 21 2
Unit cell lengths	71.179, 71.179, 173.370
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	55.070 - 1.400
R-factor	0.15411
Rwork	0.152
R-free	0.20035
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4f33 1i7z
RMSD bond length	0.013
RMSD bond angle	1.581
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	55.700	1.420
High resolution limit [Å]	1.400	1.400
Number of reflections	694016	4317
<I/σ(I)>	10.3
Completeness [%]	99.6
Redundancy	7.9
CC(1/2)	0.986	0.646

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		292	20% PEG3350 0.2 M ammonium citrate