7OVC

Structure of the human UFC1 protein in complex with the UBA5 C-terminal UFC1-binding motif.

Spectrometer

Experimental method: SOLUTION NMR

Spectrometer ID	Spectrometer maker	Spectrometer model	Spectrometer type	Spectrometer field strength
1	Bruker	AVANCE III HD		700
2	Bruker	AVANCE III HD		800
3	Bruker	AVANCE NEO		900
4	Bruker	AVANCE III		950
5	Bruker	AVANCE II		600

Experiment

experiment id	conditions id	solution id	Experiment type
1	1	1	3D [15N,1H]-TROSY-(H)C(CC)(CO)NH-TOCSY
10	1	2	3D BEST-[15N,1H]-TROSY-HNCA
11	1	2	3D BEST-[15N,1H]-TROSY-HNCACB
12	1	2	3D F1-13C/15N-filtered NOESY-[13C,1H]-HSQC
13	1	2	3D [15N,1H]-TROSY-(H)C(CC)(CO)NH-TOCSY
14	1	2	3D NOESY [13C,1H]-HSQC
15	1	2	3D [15N,1H]-TROSY-H(CC)(CO)NH-TOCSY
16	1	2	3D NOESY-[15N,1H]-SOFAST-HMQC
2	1	1	3D [15N,1H]-TROSY-H(C)(CC)(CO)NH-TOCSY
3	1	1	3D BEST-[15N,1H]-TROSY-HNCACB
4	1	1	3D F1-13C/15N-filtered NOESY-[13C,1H]-HSQC
5	1	1	3D F1-13C/15N-filtered NOESY-[15N,1H]-SOFAST-HMQC
6	1	1	3D F1-13C/15N-filtered NOESY-[13C,1H]-SOFAST-HMQC (aro)
7	1	1	3D NOESY-[13C,1H]-SOFAST-HMQC (aro)
8	1	1	3D NOESY-[13C,1H]-HSQC (aliphatic region)
9	1	1	3D NOESY-BEST-[15N,1H]-TROSY

NMR Sample

conditions id	NMR sample pH	NMR sample pressure	NMR sample temperature
1	7.5	AMBIENT	298

Conformers

Conformers Calculated Total Number	200
Conformers Submitted Total Number	20