7OTT
Metabolon-embedded pyruvate dehydrogenase complex E2 core at near-atomic resolution
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK IV |
EM Data Collection
| Microscope | TFS GLACIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 200 |
| Cs (mm) | 2.7 |
| Detector | FEI FALCON III (4k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 3.84 |
| Number of Particle | 296779 |
| Software | RELION,Coot,EPU,Gctf,UCSF ChimeraX,PHENIX |
| Symmetry | I |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
