7OTI
Structure of ABCB1/P-glycoprotein in apo state
EM specimen
| Vitrification | Yes |
EM Data Collection
| Microscope | FEI TITAN KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Phase Plate | VOLTA PHASE PLATE |
| Detector | GATAN K2 SUMMIT (4k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 4.2 |
| Number of Particle | 317000 |
| Software | cisTEM,CTFFIND |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
