7OPE
RqcH DR variant bound to 50S-peptidyl-tRNA-RqcP RQC complex (rigid body refinement)
EM specimen
Vitrification | Yes |
Instrument | FEI VITROBOT MARK III |
EM Data Collection
Microscope | FEI TITAN KRIOS |
Electron Gun | FIELD EMISSION GUN |
Accelerating Voltage (kV) | 300 |
Detector | GATAN K2 SUMMIT (4k x 4k) |
3D Reconstruction
Method | SINGLE PARTICLE |
Resolution (Å) | 3.2 |
Number of Particle | 145631 |
Software | RELION,CTFFIND |
CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |