7OMF

Structure of a minimal SF3B core in complex with sudemycin D6 (form I)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-08-29
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	106.355, 109.846, 253.300
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.030 - 3.000
R-factor	0.2341
Rwork	0.232
R-free	0.26610
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5ife 6en4
RMSD bond length	0.003
RMSD bond angle	0.604
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.19_4092)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.030	2.970
High resolution limit [Å]	2.900	2.900
Rmerge	0.086	4.094
Rmeas	0.090	4.249
Rpim	0.024	1.131
Number of reflections	66573	4382
<I/σ(I)>	19.1	0.7
Completeness [%]	99.9	98.9
Redundancy	13.7	13.8
CC(1/2)	1.000	0.389

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277.15	0.1M Bis-tris propane pH 7.4, 0.2M KSCN, 12% (w/v) PEG-3350, 10%(v/v) Glycerol