7OIH
Glycosylation in the crystal structure of neutrophil myeloperoxidase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 1 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-07-05 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.97857 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 155.910, 144.634, 236.454 |
Unit cell angles | 90.00, 91.53, 90.00 |
Refinement procedure
Resolution | 38.994 - 2.603 |
Rwork | 0.179 |
R-free | 0.21950 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6bmt |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0349) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 2.760 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmerge | 0.164 | 1.250 |
Rmeas | 0.195 | 1.470 |
Number of reflections | 158296 | 24289 |
<I/σ(I)> | 7.47 | 1.02 |
Completeness [%] | 98.7 | 95.5 |
Redundancy | 3.4 | 3.45 |
CC(1/2) | 0.990 | 0.409 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 291 | 0.2 M Potassium thiocyanate 0.1 M Sodium cacodylate 8% w/v PGA L/M |