Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-11-09 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.900 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 122.040, 122.040, 257.410 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 61.095 - 2.880 |
| Rwork | 0.182 |
| R-free | 0.27340 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Phaser |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.606 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 66.610 | 2.950 |
| High resolution limit [Å] | 2.880 | 2.880 |
| Rmerge | 0.104 | 1.803 |
| Rmeas | 0.115 | 2.019 |
| Rpim | 0.037 | 0.649 |
| Number of reflections | 51118 | 3710 |
| <I/σ(I)> | 13.5 | 1.2 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 9.8 | 9.6 |
| CC(1/2) | 0.998 | 0.529 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.2 | 289 | 18% PEG 12000 0.1M Na/K phosphate pH 6.2 0.2M NaCl 4mM DTT in the presence of 3-fold molar excess of IP6. |
