7OGB
GTPase HRAS under 500 MPa pressure
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID09 |
| Synchrotron site | ESRF |
| Beamline | ID09 |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Collection date | 2015-07-02 |
| Detector | MAR555 FLAT PANEL |
| Wavelength(s) | 0.415 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 38.635, 38.635, 166.141 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 1.850 |
| R-factor | 0.18 |
| Rwork | 0.177 |
| R-free | 0.23600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDBID 5P21 |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.970 |
| Data reduction software | XDS |
| Data scaling software | SCALA (3.3.22) |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 55.380 | 1.950 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.691 | |
| Number of reflections | 13092 | 1848 |
| <I/σ(I)> | 4.4 | 0.9 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 4.9 | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | BATCH MODE | 7.5 | 298 | 40 mM Tris HCL, 10 mM MgCl2, 2 mM DTE, 26-30 % PEG-400 |
