7OG0

Nontypeable Haemophillus influenzae SapA in open and closed conformations, in complex with double stranded RNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-08-08
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.920
Spacegroup name	P 21 21 2
Unit cell lengths	143.260, 147.970, 59.550
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	65.740 - 2.610
R-factor	0.1991
Rwork	0.197
R-free	0.24880
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3m8u
RMSD bond length	0.007
RMSD bond angle	1.175
Data reduction software	XDS
Data scaling software	xia2
Phasing software	PHASER (2.5.6)
Refinement software	REFMAC (5.8.0230)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	65.740	65.740	2.680
High resolution limit [Å]	2.610	11.670	2.610
Rmerge	0.078	0.026	0.588
Rmeas	0.102	0.035	0.764
Total number of observations	144976
Number of reflections	38239	771	5300
<I/σ(I)>	8.27	28.46	1.5
Completeness [%]	97.4	91.1	96.3
Redundancy	3.8	2.196	2.068
CC(1/2)	0.995	0.997	0.563

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		293	22% (w/v) PEG 3350, 0.25 M NaBr, 0.1 M Bis-Tris Propane pH 7.5