7OD1

Crystal structure of RBR ubiquitin ligase ARIH2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-06-14
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.28097
Spacegroup name	P 1 21 1
Unit cell lengths	77.570, 80.540, 92.090
Unit cell angles	90.00, 105.76, 90.00

Refinement procedure

Resolution	74.650 - 2.450
R-factor	0.2224
Rwork	0.220
R-free	0.26170
Structure solution method	SAD
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	74.650	2.538
High resolution limit [Å]	2.450	2.450
Number of reflections	75253	2974
<I/σ(I)>	19.5
Completeness [%]	96.0
Redundancy	18.6
CC(1/2)	0.999	0.565

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	0.2 M Sodium nitrate, 0.1 M Bis-Tris propane pH 8.5, 20 % PEG 3350