7O9J
Crystal structure of DyP-type peroxidase from Dictyostelium discoideum in complex with an activated form of oxygen
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2012-12-01 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.91985 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 141.030, 141.030, 95.560 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.250 - 1.700 |
R-factor | 0.1471 |
Rwork | 0.146 |
R-free | 0.16530 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2iiz |
RMSD bond length | 0.006 |
RMSD bond angle | 1.098 |
Data reduction software | XDS |
Data scaling software | SADABS |
Phasing software | PHASER |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.780 | 1.790 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.031 | 0.360 |
Number of reflections | 105810 | 14967 |
<I/σ(I)> | 18.46 | 2.85 |
Completeness [%] | 100.0 | 100 |
Redundancy | 26.6 | 25.44 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 7 | 293 | 2.4 M sodium malonate pH 7.0 |