7O4G
Human phosphomannomutase 2 (PMM2) wild-type soaked with the activator glucose 1,6-bisphosphate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALBA BEAMLINE XALOC |
Synchrotron site | ALBA |
Beamline | XALOC |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-05-19 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97926 |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 71.144, 71.144, 361.266 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 60.210 - 2.660 |
R-factor | 0.2261 |
Rwork | 0.224 |
R-free | 0.27300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7o0c |
RMSD bond length | 0.009 |
RMSD bond angle | 1.033 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (v1.19) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 361.270 | 2.790 |
High resolution limit [Å] | 2.660 | 2.660 |
Rmerge | 0.417 | 3.011 |
Rpim | 0.085 | 0.813 |
Number of reflections | 16779 | 2150 |
<I/σ(I)> | 9.2 | 1.8 |
Completeness [%] | 100.0 | 100 |
Redundancy | 25.3 | 26.7 |
CC(1/2) | 0.996 | 0.625 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | Drops made by mixing 1 microliter of protein solution and 1 microliter of crystallization deposit solution. Protein solution contained 5 mg/ml protein in 20 mM Hepes pH 7.5 and 0.2 M NaCl. Crystallization solution contained 0.3-0.4 M MgCl2, 24% PEG3350 and 0.1 M HEPES pH 7.5. Crystals were soaked for 5-10 min in mother liquor with 6 mM glucose 1,6-bisphophate. |