7O35
Crystal Structure of SARS-CoV-2 N-CTD in complex with GTP (I)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-11-19 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97926 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 43.773, 92.719, 68.728 |
| Unit cell angles | 90.00, 90.01, 90.00 |
Refinement procedure
| Resolution | 68.730 - 1.800 |
| R-factor | 0.1581 |
| Rwork | 0.156 |
| R-free | 0.20330 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6wzo |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.937 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.5.1) |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 92.720 | 92.720 | 1.840 |
| High resolution limit [Å] | 1.800 | 9.000 | 1.800 |
| Rmerge | 0.066 | 0.037 | 0.395 |
| Rmeas | 0.071 | 0.041 | 0.430 |
| Rpim | 0.027 | 0.016 | 0.169 |
| Total number of observations | 341444 | 2616 | 18601 |
| Number of reflections | 50472 | 430 | 2926 |
| <I/σ(I)> | 17.8 | 37.9 | 4.9 |
| Completeness [%] | 99.4 | 99.6 | 97.8 |
| Redundancy | 6.8 | 6.1 | 6.4 |
| CC(1/2) | 0.999 | 0.998 | 0.961 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 4.6 | 294 | 30% PEG 3350 0.1 M sodium acetate pH 4.6 |
