7O2E
Crystal structure of the human METTL3-METTL14 complex bound to Compound 21 (ADO_AD_089)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-12-05 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 1 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 63.930, 63.930, 224.600 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 44.520 - 2.500 |
| R-factor | 0.209 |
| Rwork | 0.206 |
| R-free | 0.27300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7acd |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.067 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19.1_4122) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.000 | 2.650 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Number of reflections | 19442 | 3082 |
| <I/σ(I)> | 17.13 | |
| Completeness [%] | 99.9 | |
| Redundancy | 8.14 | |
| CC(1/2) | 0.999 | 0.496 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 295 | 20% PEG 3350, 400mM Mg acetate |
