7O1B
Human phosphomannomutase 2 (PMM2) wild-type co-crystallized with the activator glucose 1,6-bisphosphate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE MASSIF-1 |
Synchrotron site | ESRF |
Beamline | MASSIF-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-03-11 |
Detector | DECTRIS PILATUS 2M |
Wavelength(s) | 0.966 |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 70.960, 70.960, 364.280 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 61.450 - 3.080 |
R-factor | 0.2369 |
Rwork | 0.236 |
R-free | 0.25650 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7o0c |
RMSD bond length | 0.009 |
RMSD bond angle | 1.123 |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 61.450 | 3.129 |
High resolution limit [Å] | 3.080 | 3.080 |
Rmerge | 0.341 | 1.539 |
Rmeas | 0.350 | |
Rpim | 0.081 | 0.364 |
Number of reflections | 10988 | 514 |
<I/σ(I)> | 8.4 | 2.2 |
Completeness [%] | 99.9 | 100 |
Redundancy | 18.5 | 18.6 |
CC(1/2) | 0.997 | 0.784 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | Drops made by mixing 1 microliter of protein solution and 1 microliter of crystallization deposit solution. Protein solution contained 5 mg/ml protein and 3 mM glucose 1,6-bisphophate in 20 mM Hepes pH 7.5 and 0.2 M NaCl. Crystallization solution contained 0.3-0.4 M MgCl2, 24% PEG3350 and 0.1 M HEPES pH 7.5 |