7O0R
Crystal structure of the human METTL3-METTL14 complex bound to Compound 15 (ADO_AE_026)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-02-25 |
Detector | DECTRIS PILATUS 2M-F |
Wavelength(s) | 1 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 64.050, 64.050, 225.140 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 49.760 - 2.300 |
R-factor | 0.1971 |
Rwork | 0.194 |
R-free | 0.24730 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7acd |
RMSD bond length | 0.008 |
RMSD bond angle | 0.962 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.19.1_4122) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.800 | 2.440 |
High resolution limit [Å] | 2.300 | 2.300 |
Number of reflections | 24928 | 3934 |
<I/σ(I)> | 15.27 | |
Completeness [%] | 100.0 | |
Redundancy | 8 | |
CC(1/2) | 0.998 | 0.756 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 295 | 20% PEG 3350, 400mM Mg acetate |