7O0G

Structure of the foamy viral protease-reverse transcriptase in complex with RNA/DNA hybrid.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-08-26
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.979310
Spacegroup name	P 43 21 2
Unit cell lengths	105.028, 105.028, 253.525
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.520 - 3.100
R-factor	0.2487
Rwork	0.247
R-free	0.27300
Structure solution method	SAD
RMSD bond length	0.003
RMSD bond angle	0.579
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	CRANK2
Refinement software	PHENIX (1.19_4092)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.280
High resolution limit [Å]	3.100	3.100
Number of reflections	26673	4179
<I/σ(I)>	21.1
Completeness [%]	99.8	99.3
Redundancy	13.1	13.5
CC(1/2)	0.998	0.821

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	291	PEG 20000, PEG MME550, MES/imidazole