7O0G
Structure of the foamy viral protease-reverse transcriptase in complex with RNA/DNA hybrid.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1) |
Synchrotron site | PETRA III, EMBL c/o DESY |
Beamline | P13 (MX1) |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-08-26 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.979310 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 105.028, 105.028, 253.525 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.520 - 3.100 |
R-factor | 0.2487 |
Rwork | 0.247 |
R-free | 0.27300 |
Structure solution method | SAD |
RMSD bond length | 0.003 |
RMSD bond angle | 0.579 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | CRANK2 |
Refinement software | PHENIX (1.19_4092) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.280 |
High resolution limit [Å] | 3.100 | 3.100 |
Number of reflections | 26673 | 4179 |
<I/σ(I)> | 21.1 | |
Completeness [%] | 99.8 | 99.3 |
Redundancy | 13.1 | 13.5 |
CC(1/2) | 0.998 | 0.821 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 291 | PEG 20000, PEG MME550, MES/imidazole |