Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-02-05 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.918007 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 72.100, 129.020, 50.100 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.569 - 1.998 |
| Rwork | 0.206 |
| R-free | 0.25260 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1alu |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.647 |
| Data reduction software | XDS |
| Data scaling software | pointless |
| Phasing software | PHASER (2.5.7) |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.120 |
| High resolution limit [Å] | 1.998 | 1.998 |
| Rmerge | 0.070 | 1.412 |
| Rmeas | 0.084 | 1.670 |
| Number of reflections | 16028 | 2465 |
| <I/σ(I)> | 10.62 | 0.72 |
| Completeness [%] | 98.2 | 95.9 |
| Redundancy | 3.22 | 3.38 |
| CC(1/2) | 0.999 | 0.434 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 0.2-0.25 M sodium malonate, pH 7.0 and 18-22% (w/v) PEG 3350 |
