7NXV

Crystal structure of the complex of DNase I/G-actin/PPP1R15A_582-621

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-03-31
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	0.9762
Spacegroup name	P 21 21 21
Unit cell lengths	86.519, 107.807, 192.412
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	55.110 - 2.550
R-factor	0.2175
Rwork	0.216
R-free	0.24780
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2a42
RMSD bond length	0.002
RMSD bond angle	1.209
Data reduction software	xia2
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	55.120	2.620
High resolution limit [Å]	2.550	2.550
Rmerge	0.315	1.701
Number of reflections	59509	4573
<I/σ(I)>	6.5	1.4
Completeness [%]	100.0	100
Redundancy	13.2	13.8
CC(1/2)	0.987	0.631

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	5	293	10% PEG4000, 0.1M Acetate