7NWU
Co-crystal structure of UPF3B-RRM-NOPS-L with UPF2-MIF4GIII
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04-1 |
Synchrotron site | Diamond |
Beamline | I04-1 |
Temperature [K] | 277 |
Detector technology | PIXEL |
Collection date | 2020-06-26 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.9795 |
Spacegroup name | P 41 2 2 |
Unit cell lengths | 130.593, 130.593, 267.330 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 - 2.600 |
R-factor | 0.2187 |
Rwork | 0.217 |
R-free | 0.25590 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1uw4 |
RMSD bond length | 0.004 |
RMSD bond angle | 0.536 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.19.2-4158) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 30.000 | 30.000 | 2.550 |
High resolution limit [Å] | 1.309 | 12.750 | 2.500 |
Rpim | 0.061 | 0.010 | 0.809 |
Number of reflections | 80565 | 678 | 4529 |
<I/σ(I)> | 10.2 | 39.1 | 1 |
Completeness [%] | 99.9 | 92.6 | 100 |
Redundancy | 14.2 | 14.1 | 14.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6 | 293.15 | 0.1M Ca acetate 0.1M MES 15% PEG 400 P 41 2 2 |