7NUH
Influenza A/California/07/2009(H1N1) endonuclease with I38T mutation in complex with orientin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-01-07 |
Detector | DECTRIS PILATUS 300K |
Wavelength(s) | 1.54187 |
Spacegroup name | P 64 2 2 |
Unit cell lengths | 73.967, 73.967, 127.575 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 45.240 - 2.200 |
R-factor | 0.1963 |
Rwork | 0.193 |
R-free | 0.26410 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6ya5 |
RMSD bond length | 0.008 |
RMSD bond angle | 1.478 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 45.240 | 45.240 | 2.260 |
High resolution limit [Å] | 2.200 | 9.840 | 2.200 |
Rmerge | 0.088 | 0.023 | 1.166 |
Rmeas | 0.095 | 0.025 | 1.325 |
Total number of observations | 74623 | ||
Number of reflections | 10985 | 157 | 770 |
<I/σ(I)> | 15.96 | 59.06 | 1.21 |
Completeness [%] | 99.2 | 94 | 98.1 |
Redundancy | 6.793 | 5.975 | 4.187 |
CC(1/2) | 0.998 | 1.000 | 0.386 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 291.15 | MDP, PEG 1000, PEG 3350, Sodium HEPES, MOPS (acid), Magnesium chloride, Calcium chloride |