7NRI
Structure of the darobactin-bound E. coli BAM complex (BamABCDE)
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK III |
EM Data Collection
| Microscope | FEI TITAN KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Cs (mm) | 2.7 |
| Energy Filter | GIF Bioquantum |
| Detector | GATAN K2 SUMMIT (4k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 3.03 |
| Number of Particle | 870023 |
| Software | cryoSPARC,SerialEM,UCSF Chimera,PHENIX |
| Symmetry | C1 |
| CTF correction | NONE |
