7NQA

Crystal structure of Nucleoporin-98 nanobody MS98-6 complex solved at 2.2A resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-11-12
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.000
Spacegroup name	P 21 21 21
Unit cell lengths	83.760, 96.630, 100.870
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.690 - 2.200
R-factor	0.1789
Rwork	0.176
R-free	0.22600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5e0q
RMSD bond length	0.007
RMSD bond angle	0.806
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.300	2.050
High resolution limit [Å]	2.000	2.000
Number of reflections	49105	1808
<I/σ(I)>	17.9
Completeness [%]	87.7	44
Redundancy	13.2	4.98
CC(1/2)	0.990	0.320

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	9	291	2.5% (w/v) PEG 6000, 25% (v/v) PEG MME 500, 0.1 M Tris-HCl pH 9.0