7NQ4

Human tRNA guanine transglycosylase (TGT), RNA-bound covalent intermediate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	93.15
Detector technology	PIXEL
Collection date	2020-02-28
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.918400
Spacegroup name	C 1 2 1
Unit cell lengths	161.840, 56.960, 102.960
Unit cell angles	90.00, 124.94, 90.00

Refinement procedure

Resolution	42.200 - 2.880
R-factor	0.2135
Rwork	0.212
R-free	0.24840
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6h42 6fv5
RMSD bond length	0.007
RMSD bond angle	1.414
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER (v2.8.3)
Refinement software	REFMAC (v5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.200	2.980
High resolution limit [Å]	2.880	2.880
Rmeas	0.116
Number of reflections	17591	1703
<I/σ(I)>	13.83	1.81
Completeness [%]	81.6	99.8
Redundancy	6.788	6.985
CC(1/2)	0.999	0.876

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277.15	PEG 1500, MMT pH 6