7NPW
Cryo-EM structure of Human excitatory amino acid transporters-1 (EAAT1) in potassium buffer
EM specimen
Vitrification | Yes |
Instrument | FEI VITROBOT MARK IV |
EM Data Collection
Microscope | FEI TITAN KRIOS |
Electron Gun | FIELD EMISSION GUN |
Accelerating Voltage (kV) | 300 |
Cs (mm) | 2.7 |
Detector | GATAN K2 SUMMIT (4k x 4k) |
3D Reconstruction
Method | SINGLE PARTICLE |
Resolution (Å) | 3.99 |
Number of Particle | 2298481 |
Software | cryoSPARC,PHENIX,SerialEM,Gctf,UCSF Chimera,Coot |
Symmetry | C3 |
CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |