7NP9

Structure of the human CR3 - CD11bCD18 specific nanobody hCR3Nb1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P14 (MX2)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-11-22
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.976300
Spacegroup name	I 41 2 2
Unit cell lengths	82.781, 82.781, 72.456
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	54.520 - 1.140
R-factor	0.1472
Rwork	0.146
R-free	0.16690
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6ehg
RMSD bond length	0.010
RMSD bond angle	1.163
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.19rc5_4047)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	54.520	1.181
High resolution limit [Å]	1.140	1.140
Rmerge	0.159
Number of reflections	44396	3194
<I/σ(I)>	21.5
Completeness [%]	96.4
Redundancy	45
CC(1/2)	1.000	0.807

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		292	1.5 M AmSO4, 0.1 M BIS-TRIS pH 6.5