7NOX
Structure of SGBP BO2743 from Bacteroides ovatus in complex with mixed-linked gluco-nonasaccharide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I02 |
Synchrotron site | Diamond |
Beamline | I02 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-07-26 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.9795 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 87.842, 88.437, 156.148 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 44.860 - 1.430 |
Rwork | 0.162 |
R-free | 0.18970 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5j5u |
Data reduction software | STARANISO |
Data scaling software | STARANISO |
Phasing software | PHASER |
Refinement software | PHENIX (1.19) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 62.320 | 1.550 |
High resolution limit [Å] | 1.430 | 1.430 |
Number of reflections | 175289 | 8764 |
<I/σ(I)> | 1.6 | |
Completeness [%] | 96.5 | |
Redundancy | 9 | |
CC(1/2) | 1.000 | 0.740 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | Protein at 8 mg/mL; 25% PEG 3350, 0.1 M Bis-Tris pH 5.5, 0.2 mM MgCl2.6H2O |