7NLV

WILDTYPE CORE-STREPTAVIDIN WITH a conjugated BIOTINYLATED PYRROLIDINE II

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-10-25
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	0.915870
Spacegroup name	P 1 21 1
Unit cell lengths	49.320, 97.600, 50.900
Unit cell angles	90.00, 110.26, 90.00

Refinement procedure

Resolution	46.270 - 1.290
Rwork	0.207
R-free	0.24710
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6gh7
RMSD bond length	0.010
RMSD bond angle	1.603
Data reduction software	Aimless (0.7.4)
Data scaling software	xia2
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	46.270	46.270	1.310
High resolution limit [Å]	1.290	7.070	1.290
Rmerge	0.052	0.023	0.490
Rmeas	0.073	0.032	0.694
Rpim	0.052	0.023	0.490
Number of reflections	112641	699	5593
<I/σ(I)>	7.3
Completeness [%]	99.5
Redundancy	1.9	1.8	1.9
CC(1/2)	0.994	0.995	0.596

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	25 mg/mL protein in deionised water is mixed at equal volume with 52% MPD.