7NLV
WILDTYPE CORE-STREPTAVIDIN WITH a conjugated BIOTINYLATED PYRROLIDINE II
This is a non-PDB format compatible entry.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-10-25 |
Detector | DECTRIS EIGER2 XE 16M |
Wavelength(s) | 0.915870 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 49.320, 97.600, 50.900 |
Unit cell angles | 90.00, 110.26, 90.00 |
Refinement procedure
Resolution | 46.270 - 1.290 |
Rwork | 0.207 |
R-free | 0.24710 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6gh7 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.603 |
Data reduction software | Aimless (0.7.4) |
Data scaling software | xia2 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 46.270 | 46.270 | 1.310 |
High resolution limit [Å] | 1.290 | 7.070 | 1.290 |
Rmerge | 0.052 | 0.023 | 0.490 |
Rmeas | 0.073 | 0.032 | 0.694 |
Rpim | 0.052 | 0.023 | 0.490 |
Number of reflections | 112641 | 699 | 5593 |
<I/σ(I)> | 7.3 | ||
Completeness [%] | 99.5 | ||
Redundancy | 1.9 | 1.8 | 1.9 |
CC(1/2) | 0.994 | 0.995 | 0.596 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 25 mg/mL protein in deionised water is mixed at equal volume with 52% MPD. |