7NLC

Crystallographic structure of human Tsg101 UEV domain in complex with a HEV ORF3 peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 2
Synchrotron site	SOLEIL
Beamline	PROXIMA 2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-10-09
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.980108
Spacegroup name	P 41 21 2
Unit cell lengths	42.570, 42.570, 188.160
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.084 - 1.398
Rwork	0.220
R-free	0.23900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3obq
RMSD bond length	0.013
RMSD bond angle	1.786
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.084	1.480
High resolution limit [Å]	1.398	1.400
Number of reflections	35532	5587
<I/σ(I)>	23.7
Completeness [%]	100.0
Redundancy	4.6
CC(1/2)	1.000	0.875

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	3.5	288	0.1M Citric Acid, 2M Ammonium sulfate pH 3.5, 13.34% glycerol