7NL6
Crystal Structure of DC-SIGN in complex with a triazole-based glycomimetic ligand
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-08-26 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.00004 |
| Spacegroup name | P 3 2 1 |
| Unit cell lengths | 59.473, 59.473, 73.939 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 42.262 - 2.200 |
| R-factor | 0.1958 |
| Rwork | 0.194 |
| R-free | 0.22920 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1sl4 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.262 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.295 | 1.750 |
| Rpim | 0.084 | 0.490 |
| Number of reflections | 8051 | 795 |
| <I/σ(I)> | 6.5 | 1.1 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 13.1 | 13.3 |
| CC(1/2) | 0.991 | 0.468 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 20.5% PEG 3350 and 0.05M NH4NO3 |
