7NK0

Structure of the BIR1 domain of cIAP2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	80
Detector technology	PIXEL
Collection date	2017-04-27
Detector	DECTRIS PILATUS3 R CdTe 300K
Wavelength(s)	0.984
Spacegroup name	P 64
Unit cell lengths	93.430, 93.430, 42.430
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	46.720 - 3.300
R-factor	0.2978
Rwork	0.295
R-free	0.35580
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3m0a
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.720	3.390
High resolution limit [Å]	3.300	3.300
Number of reflections	3285	3291
<I/σ(I)>	1.3
Completeness [%]	99.9
Redundancy	20
CC(1/2)	0.770	0.600

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	12 % w/v Polyethylene glycol 8,000, 10 % w/v Glycerol, 500 mM Potassium chloride