7NIY
E. coli NfsA with FMN
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-10-18 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.934 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 91.911, 52.003, 64.695 |
| Unit cell angles | 90.00, 133.78, 90.00 |
Refinement procedure
| Resolution | 32.360 - 1.030 |
| R-factor | 0.1056 |
| Rwork | 0.105 |
| R-free | 0.11830 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1fv5 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.752 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.360 | 1.056 |
| High resolution limit [Å] | 1.030 | 1.030 |
| Number of reflections | 99892 | 7069 |
| <I/σ(I)> | 27.89 | |
| Completeness [%] | 96.7 | 92.3 |
| Redundancy | 8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 291 | 30 % PEG 3,350; 100 mM Imidazole, 20 mM Hydroquinone, 15 % Ethylene Glycol |
