7NH2
Crystal structure of Toxoplasma CPSF4-YTH domain bound to m6A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE MASSIF-1 |
| Synchrotron site | ESRF |
| Beamline | MASSIF-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-09-26 |
| Detector | DECTRIS PILATUS3 2M |
| Wavelength(s) | 0.9660 |
| Spacegroup name | P 1 |
| Unit cell lengths | 32.474, 35.221, 38.177 |
| Unit cell angles | 114.51, 100.44, 97.77 |
Refinement procedure
| Resolution | 30.760 - 1.350 |
| R-factor | 0.1832 |
| Rwork | 0.182 |
| R-free | 0.19670 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4r3i |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.822 |
| Data reduction software | xia2 |
| Data scaling software | DIALS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.760 | 1.397 |
| High resolution limit [Å] | 1.349 | 1.349 |
| Number of reflections | 30036 | 2887 |
| <I/σ(I)> | 6.6 | 2.1 |
| Completeness [%] | 92.6 | 88.38 |
| Redundancy | 1.6 | 1.7 |
| CC(1/2) | 0.956 | 0.496 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 293 | 50% PEG 400, 0.1M Sodium acetate pH 4.5 and 0.2M LiSO4 |
