7NFT

Fujian capbinding domain in complex with Nb8208

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-05-14
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.9763
Spacegroup name	P 6
Unit cell lengths	128.195, 128.195, 70.266
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	70.270 - 3.140
R-factor	0.2463
Rwork	0.244
R-free	0.29140
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6s5v
RMSD bond length	0.002
RMSD bond angle	0.498
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.19.1_4122)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	70.270	3.256
High resolution limit [Å]	3.140	3.144
Number of reflections	10820	377
<I/σ(I)>	9.85
Completeness [%]	92.9
Redundancy	2
CC(1/2)	0.989	0.595

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	20% w/v PEG3350, 1 M (NH4)2SO4, 0.1M BIS-TRIS propane pH 5.5