7NFR

Fujian capmidlink domain in complex with Nb8194

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-05-14
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.97625
Spacegroup name	P 1 21 1
Unit cell lengths	55.596, 65.462, 59.990
Unit cell angles	90.00, 108.22, 90.00

Refinement procedure

Resolution	56.980 - 1.880
R-factor	0.1974
Rwork	0.195
R-free	0.24510
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6s5v
RMSD bond length	0.005
RMSD bond angle	0.572
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.18rc2_3794)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	56.980	1.950
High resolution limit [Å]	1.880	1.880
Number of reflections	30100	1775
<I/σ(I)>	9.29
Completeness [%]	89.9
Redundancy	2
CC(1/2)	1.000	0.159

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	25% w/v Peg3350, 0.2 M (NH4)2SO4, and 0.1 M Tris pH 8.5