7NEE
Inhibitor Complex with Thrombin Activatable Fibrinolysis inhibitor (TAFIa)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-12-07 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.03324 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 64.170, 95.500, 157.220 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.384 - 2.550 |
| Rwork | 0.254 |
| R-free | 0.31560 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | in house model |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.860 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.384 | 2.660 |
| High resolution limit [Å] | 2.550 | 2.550 |
| Number of reflections | 15499 | 1862 |
| <I/σ(I)> | 9.5 | |
| Completeness [%] | 96.2 | |
| Redundancy | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 23% PEG 8000, 200mM Ammonium Citrate, 100 mM MES |
