7NDL

Crystal structure of human GFAT-1 S205D

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	80
Detector technology	PIXEL
Collection date	2020-12-04
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9747
Spacegroup name	P 41 21 2
Unit cell lengths	153.615, 153.615, 166.132
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	90.914 - 2.223
R-factor	0.1765
Rwork	0.176
R-free	0.19340
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6r4e
Data reduction software	XDS (Jan 31, 2020)
Data scaling software	XDS (Jan 31, 2020)
Phasing software	PHENIX
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	90.914	2.303
High resolution limit [Å]	2.223	2.223
Number of reflections	97303	9193
<I/σ(I)>	22.99
Completeness [%]	99.5
Redundancy	25.8
CC(1/2)	1.000	0.581

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293.15	0.1 M Bis tris propane pH 9.0, 0.3 M Sodium malonate dibasic, 20 % PEG3350