7N96
Crystal structure of D103A human Galectin-7 mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08B1-1 |
| Synchrotron site | CLSI |
| Beamline | 08B1-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2019-02-19 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 1.0332 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 53.510, 67.110, 68.010 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.770 - 2.380 |
| R-factor | 0.2221 |
| Rwork | 0.218 |
| R-free | 0.25940 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1bkz |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.882 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER (2.7.12) |
| Refinement software | PHENIX (1.19.2-4158) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 47.770 | 47.780 | 2.470 |
| High resolution limit [Å] | 2.380 | 5.130 | 2.380 |
| Rmerge | 0.154 | 0.046 | 1.520 |
| Rmeas | 0.163 | 0.048 | 1.603 |
| Rpim | 0.052 | 0.016 | 0.502 |
| Number of reflections | 10282 | 1111 | 1003 |
| <I/σ(I)> | 13.7 | 35.8 | 1.9 |
| Completeness [%] | 100.0 | 100 | 99.8 |
| Redundancy | 9.6 | 8.8 | 9.8 |
| CC(1/2) | 0.998 | 0.999 | 0.719 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 295.15 | 0.1 M NaCl, 0.1 M Tris pH 8, 20 % PEG 6000 ,15% Glycerol |
