7N8M
PptT PAP(CoA) 8978B complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 120 |
Detector technology | PIXEL |
Collection date | 2017-08-06 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.98 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 134.759, 63.709, 79.669 |
Unit cell angles | 90.00, 123.33, 90.00 |
Refinement procedure
Resolution | 39.790 - 1.570 |
R-factor | 0.1727 |
Rwork | 0.172 |
R-free | 0.20170 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6ct5 |
RMSD bond length | 0.009 |
RMSD bond angle | 0.993 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHASER |
Refinement software | PHENIX (dev_3126) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 39.790 | 1.625 |
High resolution limit [Å] | 1.570 | 1.570 |
Number of reflections | 78467 | 7824 |
<I/σ(I)> | 27.8 | |
Completeness [%] | 99.6 | 99.48 |
Redundancy | 4.9 | |
CC(1/2) | 0.949 | 0.779 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.7 | 291.15 | 0.1 M Bis-Tris Propane pH 6.7, 1.8M Magnesium Sulfate |