7N8M

PptT PAP(CoA) 8978B complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	120
Detector technology	PIXEL
Collection date	2017-08-06
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.98
Spacegroup name	C 1 2 1
Unit cell lengths	134.759, 63.709, 79.669
Unit cell angles	90.00, 123.33, 90.00

Refinement procedure

Resolution	39.790 - 1.570
R-factor	0.1727
Rwork	0.172
R-free	0.20170
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6ct5
RMSD bond length	0.009
RMSD bond angle	0.993
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX (dev_3126)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.790	1.625
High resolution limit [Å]	1.570	1.570
Number of reflections	78467	7824
<I/σ(I)>	27.8
Completeness [%]	99.6	99.48
Redundancy	4.9
CC(1/2)	0.949	0.779

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.7	291.15	0.1 M Bis-Tris Propane pH 6.7, 1.8M Magnesium Sulfate