7N8K

LINE-1 endonuclease domain complex with Mg

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	180
Detector technology	IMAGE PLATE
Collection date	2019-07-18
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.54
Spacegroup name	P 1 21 1
Unit cell lengths	42.857, 126.776, 44.854
Unit cell angles	90.00, 98.94, 90.00

Refinement procedure

Resolution	33.300 - 2.010
R-factor	0.1949
Rwork	0.192
R-free	0.23610
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1vyb
RMSD bond length	0.008
RMSD bond angle	1.099
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHENIX
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.040
High resolution limit [Å]	2.010	2.010
Rmerge	0.089	0.750
Rmeas	0.104
Rpim	0.055
Number of reflections	30980	1505
<I/σ(I)>	16.3	1.54
Completeness [%]	98.2	93.7
Redundancy	3.5	3
CC(1/2)	0.998	0.627

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	6	298	0.1M LiSO4; 26% PEG 2000 MME