7N86
Crystal Structure of Human Protocadherin-24 EC1-2 Form II
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-12-01 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 63.899, 86.641, 104.892 |
| Unit cell angles | 90.00, 103.46, 90.00 |
Refinement procedure
| Resolution | 49.147 - 3.175 |
| Rwork | 0.213 |
| R-free | 0.28960 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5czr |
| RMSD bond length | 0.017 |
| RMSD bond angle | 2.020 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.230 |
| High resolution limit [Å] | 3.175 | 3.175 |
| Number of reflections | 18809 | 810 |
| <I/σ(I)> | 5.6 | 2.63 |
| Completeness [%] | 96.3 | 89.1 |
| Redundancy | 3.5 | 2.5 |
| CC(1/2) | 0.987 | 0.277 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 277 | 0.2 MM NaI 20% PEG3350 CDHR5 EC1-2 |
