7N6C
Crystal structure of R22A human Galectin-7 mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CLSI BEAMLINE 08B1-1 |
Synchrotron site | CLSI |
Beamline | 08B1-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2019-02-19 |
Detector | RAYONIX MX300HE |
Wavelength(s) | 1.0332 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 53.350, 64.890, 71.900 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.170 - 2.100 |
R-factor | 0.201 |
Rwork | 0.197 |
R-free | 0.23500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1bkz |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER (2.7.12) |
Refinement software | PHENIX (1.19.2-4158) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 48.190 | 48.190 | 2.120 |
High resolution limit [Å] | 2.050 | 4.420 | 2.050 |
Rmerge | 0.120 | 0.050 | 1.371 |
Rmeas | 0.127 | 0.052 | 1.443 |
Rpim | 0.017 | ||
Number of reflections | 16194 | 1740 | 1570 |
<I/σ(I)> | 12.8 | 34.3 | 1.8 |
Completeness [%] | 99.9 | 99.8 | 99.8 |
Redundancy | 10.1 | 9.4 | 10.2 |
CC(1/2) | 0.998 | 0.998 | 0.685 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 295.15 | 0.1 M NaCl, 0.1 M Tris pH 8, 20 % PEG 6000, 17.5% Glycerol |