7N4O
Crystal structure of R20A human Galectin-7 mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CLSI BEAMLINE 08B1-1 |
Synchrotron site | CLSI |
Beamline | 08B1-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2019-02-19 |
Detector | RAYONIX MX300HE |
Wavelength(s) | 1.0332 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 53.430, 64.640, 71.810 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.040 - 2.050 |
R-factor | 0.1915 |
Rwork | 0.185 |
R-free | 0.25210 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1bkz |
RMSD bond length | 0.006 |
RMSD bond angle | 0.749 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER (2.7.12) |
Refinement software | PHENIX (1.19.2-4158) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 48.040 | 48.040 | 2.000 |
High resolution limit [Å] | 1.890 | 5.000 | 1.890 |
Rmerge | 0.134 | 0.027 | 2.439 |
Rmeas | 0.145 | 0.029 | 2.627 |
Total number of observations | 148532 | ||
Number of reflections | 20513 | 1209 | 3136 |
<I/σ(I)> | 12.87 | 49.7 | 0.88 |
Completeness [%] | 100.0 | 99.7 | 100 |
Redundancy | 7.241 | 6.691 | 7.297 |
CC(1/2) | 0.998 | 1.000 | 0.324 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 295.15 | 0.1 M NaCl, 0.1 M Tris pH 8, 20 % PEG 6000 , 20% Glycerol |