7N3D
Crystal Structure of Human Fab S24-1564 in the complex with the N-terminal Domain of Nucleocapsid protein from SARS CoV-2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-02-02 |
| Detector | DECTRIS PILATUS3 X 6M |
| Wavelength(s) | 0.97890 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 50.927, 67.318, 86.087 |
| Unit cell angles | 90.00, 106.64, 90.00 |
Refinement procedure
| Resolution | 39.510 - 1.530 |
| R-factor | 0.1588 |
| Rwork | 0.157 |
| R-free | 0.19020 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDBIDs 5KKU 5o4g 6ieb |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.015 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | PHENIX (1.19_4092) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.560 |
| High resolution limit [Å] | 1.520 | 1.530 |
| Rmerge | 0.114 | 0.834 |
| Number of reflections | 81619 | 2795 |
| <I/σ(I)> | 22 | 0.96 |
| Completeness [%] | 95.9 | 66.2 |
| Redundancy | 4.4 | 1.9 |
| CC(1/2) | 0.986 | 0.412 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 298 | 0.2 M Magnesium chloride hexahydrate, 0.1 M HEPES pH 7.5, 25% w/v Polyethylene glycol 3,350 |
