7N1O
The von Willebrand factor A domain of human capillary morphogenesis gene II, flexibly fused to the 1TEL crystallization chaperone
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-2 |
Synchrotron site | SSRL |
Beamline | BL9-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-10-21 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.979460 |
Spacegroup name | P 65 |
Unit cell lengths | 103.396, 103.396, 56.550 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 38.160 - 2.770 |
R-factor | 0.2045 |
Rwork | 0.203 |
R-free | 0.22840 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2qb1 1shu |
RMSD bond length | 0.009 |
RMSD bond angle | 0.856 |
Data reduction software | autoPROC |
Data scaling software | autoPROC |
Phasing software | PHASER |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 38.160 | 2.869 |
High resolution limit [Å] | 2.770 | 2.770 |
Rmerge | 0.052 | 0.625 |
Rmeas | 0.058 | 0.688 |
Rpim | 0.024 | 0.278 |
Number of reflections | 8679 | 878 |
<I/σ(I)> | 18.35 | 2.88 |
Completeness [%] | 97.1 | 97.99 |
Redundancy | 5.5 | 5.8 |
CC(1/2) | 0.999 | 0.899 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.7 | 298 | 100 mM BisTris, 3.0 M NaCl |