7N1O

The von Willebrand factor A domain of human capillary morphogenesis gene II, flexibly fused to the 1TEL crystallization chaperone

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-10-21
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.979460
Spacegroup name	P 65
Unit cell lengths	103.396, 103.396, 56.550
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	38.160 - 2.770
R-factor	0.2045
Rwork	0.203
R-free	0.22840
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2qb1 1shu
RMSD bond length	0.009
RMSD bond angle	0.856
Data reduction software	autoPROC
Data scaling software	autoPROC
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.160	2.869
High resolution limit [Å]	2.770	2.770
Rmerge	0.052	0.625
Rmeas	0.058	0.688
Rpim	0.024	0.278
Number of reflections	8679	878
<I/σ(I)>	18.35	2.88
Completeness [%]	97.1	97.99
Redundancy	5.5	5.8
CC(1/2)	0.999	0.899

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.7	298	100 mM BisTris, 3.0 M NaCl